35S启动子组入蓝藻质粒载体pUC13－pbl

Insetting of 35S promoter into the plasmid vector PUC13-pb1 of the cyanobacteria

３５Ｓ启动子组入蓝藻质粒载体ｐＵＣ１３－ｐｂｌ楼士林，郭奕斌，吴巧娟（生物学系）近年来构建了一些含３５Ｓ启动子的质粒载体￣［１］，但未见含３５Ｓ启动子的蓝藻质粒载体．因此本研究拟把ＣａＭＶ的３５Ｓ启动子组入由孙立春等人构建的蓝藻质粒载体ｐＵＣ１３－ｐ...

The p35S-CAT and puc13-pbl were digested with Bg1I resprectively.Anew recombiation plasmid pUP-35S was constructed by combining 4.1 kb DNA fragment fromp35S-CAT with 3.1kb DNAfragment from PUC13-pbl.pUP-35S contains intact small plasmidof filamentous cyanobacterium Plectonema boryanum and CaMV 35S promoter andchloramphenicol acetytransferase gene from p35S-CAT