上皮性卵巢癌中P^（53）基因点突变的初步研究

A preliminary study of point mutations of P￣(53) gene in epithelial ovarian carcinoma

为研究肿瘤抑制基因Ｐ５３与卵巢癌的关系，用多聚酶链反应－单链构象多态（ＰＣＲ－ＳＳＣＰ）银染技术检测了３０例上皮性卵巢癌中Ｐ５３基因第５～８外显子的点突变。结果；ＳＳＣＰ阳性１２例，阳性率为４０％，第５、６、７外显子分别为１、２、９例。Ｐ５３基因点突变率在各临床分期、组织学分级、组织学类型之间未发现明显差异，说明Ｐ５３基因是突变在卵巢癌的发展过程中可能一直起着重要作用。

In order to research the relationship between P53 gene and epithelial ovarian carcinoma,30 cases of ovarian carcinoma were studied using PCR-SSCP to determine the point mutations of P53 gene (exon 5～8). The results showed that 12 of 30 cancerous specimens were SSCP positive (40%). 1,2 and 9cases were occurred at exon 5, 6and 7 respectively.The point mutations of P53 gene were detected in almost all stages,histological grades, histological types as well. This study preliminarily suggests that point mutation of P53 gene may play an important role in development of ovarian carcinoma.