有机磷农药在细菌回变试验中的诱变性及其与结构的关系

THE MUTAGENICITY OF ORGANOPHOSPHORUS PESTICIDES IN BACTERIAL REVERSION ASSAY SYSTEM AND THE STRUCTURE-ACTIVITY RELATIONSHIP

采用Ａｍｅｓ试验和大肠杆菌回变试验，对１９种有机磷农药进行了诱变性研究，并分析了５９种有机磷农药化学结构与诱变性的关系。结果１９种受试有机磷农药在两种回变试验中获阳性结果的有５种，而且不论加与不加Ｓ９均不改变其定性结果。构效分析提示：三类构份的亚类改变可影响有机磷的诱变性，Ｙ为链烃、Ｘ为Ｏ或Ｓ的甲基有机磷诱发回变的概率极高，Ｘ为Ｏ时诱变性最高。推测有机磷主要攻击鸟嘌呤的Ｎ－７位，通过甲基化作用引起碱基置换型突变。

Atotal of 19 organophosphorus pesticides(OPPs)were tested for mutagenicity by Ames test and Escherichia coli(E,coli)reversion assay,and the chemical structure and mutagenicity relationship of 59 OPPs were analyzed,Five out of the 19 OPPs give positive result in both reversion assay systems and the result were unchanged no matter the S9 is added in or not.The analysis of structure-activity relationship suggested that the mutagenicity ol OPPs could be affected by the subtype patterns changes of the three constituents,The incidence of reversion induced when Y as alkane and X as O or S was high,and it mainly attacked N-7 Position of quanine, and induced bass-pair substitution mutation through methylation.