双温PCR法检测淋巴细胞白血病微小残留病

To Detect Minimal Residual Disease(MRD)in Lymphoblastic Leukemias by Two-temperature PCR Method

免疫球蛋白重链（ＩｇＨ）重排可作为Ｂ淋巴系肿瘤的基因标志，应用ＰＣＲ方法检测此重排可进行淋巴细胞白血病微小残留病（ＭＲＤ）的研究。我们通过基因释放剂提取ＤＮＡ及双温ＰＣＲ循环，建立一种快速可靠ＰＣＲ方法检测３８例淋巴细胞白血病ＩｇＨ重排基因，２８／３８（７３．７％）阳性，敏感度为１０￣（－４）～１０￣（－５）水平。此方法简便、快捷且成本低，更适于临床检测实际需要。

Immunoglobulin heavy chain(IgH)gene rearrangement serves as a marker of clonality inB lymphoproliferative malignancies. PolymeraseChain reaction(PCR)can be used to detect this rear-rangement in the research of minimal residual disease(MRD).We report a simple and rapid PCR methodby using gene releaser to extract DNA and two-tem-perature PCR cycle to detect 38 ALL and CLL pa-tients. 28 patients(73.7%)were found with IgH re-arrangement,the sensitivity was between 10￣(-4) to10￣(-50).This method is rapid,simple,low cost and de-pendable,so it is more suitable for a routine servica-ble laboratory.