摘要
用聚合酶链反应（ＰＣＲ）检测了１１４例传染科门诊病人血清ＨＢＶ－ＤＮＡ，并与ＨＢＶ有关的血清学标志（ＨＢＶＭ）进行了比较。结果表明：在９２和ＪＨＢｓＡｇ（＋）病例中，ＰＣＲ检测ＨＢＶ－ＤＮＡ阳性者５０例，占５４．３４％；在２８例ＨＢｅＡｇ（＋）病例中，ＰＣＲ检测ＨＢＶ－ＤＮＡ阳性者２６例，占９２．８６％；在２０例ＨＢＶＭ均为阴性的病例中，ＰＣＲ检测ＨＢＶ－ＤＮＡ阳性者有６例，占３０．００％。证明ＰＣＲ法检测ＨＢＶ－ＤＮＡ较用ＥＬＩＳＡ法检测抗原一抗体更加灵敏、可靠。
erum HBVDNA
was detected by polymerase chain reaction(PCR)in 114 HB outpatients from the department of
infectious diseases,First Affiliated Hospital,Xinjiang MedicalCollege。And comparison of
serum positivities of HBV-DNA by PCR and other hepatitis Bvirus markers(HBVMs)by ELISA
was made。he restilts revealed HBV-DNA positive byPCR in 50 of 92 cases with HBsAg
positive(53.34%),in 26 of 28 cases with HBeAg posi-tive(92.86%),and in 6 of 20 cases with
negative in all other HBVMs(30.00%).It sug-gested detection of HBV-DNA by PCR was more
sensitive and reliable than that by ELISA。
