对有脾、无脾小鼠D－氨基半乳糖半数致死量的测定及其意义

The Determination and Signification of LD50 of D-Galactosaminein The BALB/CMouse with Pseudosplenectomy and Splenectomy

为确定脾在小鼠急性实验性肝损伤中的作用，采用配对设计Ｋａｒｂｅｒ氏法测定了假切脾和切脾小鼠Ｄ－氨基半乳糖的半数致死量，并作尸检和活检，假切脾组半数致死量为４８６８ｍｇ／ｋｇ体重，切脾组为４５９３ｍｇ／ｋｇ体重，两者之间行Ｕ检验，结果差异有显著性。提示脾参与了小鼠机体的抗急性肝损伤机制而具有保肝作用。

n order to define the role of spleen in acute experimental hepatic injury in the BALB/Cmouse,LD50 of DGalactosamine(D-GaIN)was determined on A(pseudosplenectomy)andBgroups(splenectomy), with paired design and Karber、method.Autopsies and biopsieswere alsodone。 LD50 of Agroup was 4 868 mg/kg,and LD50 of Bgroup was 4 593 mg/kg。Utest:P<0. 05.It suggests that splel1ectomy can cut down the reserved function of liver orspleen in vivo can protect liver against D-GaIN一like materiais。