奶牛血清孕酮和总胆固醇水平与超数排卵效果的关系

RELATIONSHIP BETWEEN PROGESTERONE AND TOTAL CHOLESTEROL LEVELS IN SERUM AND THE SUPRAOVULATION RATE IN DAIRY COWS

为了研究血清中若干生化指标与母牛超数排卵的关系。在性周期中期第10天或11天超排处理之前颈静脉采血,用以测定血清中孕酮、总胆固醇等指标的水平,而后用FSH进行超排处理。输精后第7天左右采用非手术采卵,并通过形态学检查对胚胎进行鉴定。根据可用胚胎数将供体母牛分为A组(可用胚胎>2枚)和B组(可用胚胎≤2枚),并比较两组孕酮、总胆固醇等指标的水平。结果表明:周期中期血清孕酮水平在3.0ng/ml以上对于预选供体母牛是有效的。血清总胆固醇水平与可用胚胎数的相关系数r=0.6079(n=19;P<0.01),周期中期血清总胆固醇水平高于140mg/dl似乎可以做为预选供体牛较好的参数。

Blood samples were taken from 19 Black and white dairy cows before supraovulation treatment on day 10 or day 11 of estrous cycle and progesterone and total cnolesterol in sera were measured. Cows were supraovulated with FSH-PGF2a and artificially inseminated with frozen semen. According to the number of transferrable embryos collected 7 days after estrus. cows were divided into group A (>2 transferrable embryos) and group B (<= 2 transferrable embryos), and their progesterone and total cholesterol levels in sera were compared. Results showed that serum progesterone level higher than 3.0 ng/ml in mid-estrus cycle may be considered useful for selecting a donor cow. The correlation coefficient between serum total cholesterol and number of transferrable embryos Collected is 0.6079 (n = 19, P<0.01). It seems that serum total cholesterol level of higher than 140 mg/100ml can be used as a good parameter for the selection of donor cows.