摘要
我们将Ｓｈｉｂａｔａ制备ＤＮＡ的方法加以改进，采用二甲苯脱蜡、酚—氯仿—异戊醇抽提、乙醇沉淀等步骤，成功地从经甲醛固定、石蜡包埋的食管癌组织中提取出ＤＮＡ，用于聚合酶链式反应（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＰＣＲ）。结果与从新鲜冷冻组织提取的ＤＮＡ结果相似，解决了分子生物学研究中在短时间内难以得到大量新鲜肿瘤组织标本的难题。
y a modified Shibata’
s method and using the procedure that xylene dissolved
paraffin,phenol-chloroform-iso-amylalcohol denatured, and ethanol deposited nucleic acid, we
hadsuccessfully extracted DNA from formalin-fixed, paraffin-embedded tissue,and performedPCR
amplification. The result was similar to that DNA from fresh tissue,It solved the prob-lem that is
difficult to obtain many samples within limited time.
