摘要
目的试图建立光镜下突触可塑性研究新的可靠的染色方法。方法采用人或大鼠的肌肉标本固定、切片,借助组化方法建立运动终板染色法。结果本染色法能同时显示肌肉、运动终板及运动神经终末结构,并能定量各种神经末梢发芽。结论本文建立了新的神经肌肉接头染色法。本法省时、操作简单方便、重复性好,为同类研究提供了一个良好的方法。)
Objective Trying to establish a new and reliable staining of study of synaptic plasticity.Method The samples were taken from humanand rats, then fixed, sectioned and at last thestaining method of neuromuscular junction wasestablished with histochemistry method.Results The method established is allowed forvisualization of muscles, endplate and motornerve endings and all kinds of sproutings in lightmicroscope simultaneousluy. It is possible toquantitate all kinds of sproutings of motor nerveendings with this method.Conclusion The staining method establishedof neuromscular junction is simple,convenient. reliable and reproducible and worthyto be used in the same studies.
出处
《新乡医学院学报》
CAS
1995年第2期106-108,共3页
Journal of Xinxiang Medical University
基金
国家自然科学基金
关键词
突触
神经肌肉接头
运动神经末梢
银染色法
synaptic
neuromuscularjunction
motor nerve ending
cholinesterase
silver staining