用抗人结肠癌单克隆抗体检测血清和粪便中大肠癌相关抗原的研究

Study on detection of cancer associated antigen in serum and feces by monoclonal antibodies against hu-man colorectal cancer

目的 研究抗人结肠癌MAb检测血清及粪液中癌相关抗原的价值。 方法 采用抗人结肠癌单抗SC_(3A)和SC_6检测47例结直肠癌、33例结直肠腺瘤、15例增生性息肉、14例炎性息肉及60例正常人群的血清和粪便中癌相关抗原。经ELISA双抗体夹心法检测。 结果 血清和粪便阳性率在结直肠癌组分别为66.0％和72.3％;腺瘤组为63.6％和60.6％;增生性息肉组为35.7％和57.1％;炎性息肉组为40.0％和33.3％;而正常对照组仅为11.7％和13.3％。45例大肠癌Dukes分期进行检测,早期癌粪便检测的阳性率为72.2％,而血清为50.0％。 结论 ELISA法检测不仅对大肠癌及癌前病变具有较好的敏感性,而且对早期癌及癌前期病变检测较血清学检测意义更大。此外,随腺瘤不典型增生程度加重而检测阳性率增高。粪便中检测大肠癌相关抗原,方法简便,取材容易,具较好敏感性,对大规模人群普查大肠癌具有一定的应用价值。

AIM To study the value of detection of cancer associated antigens in serum and feces by using monoclonal antibodies against human col-orectal cancer.METHODS Cancer associated antigen in serum and feces was detected by using double - antibody sandwich ELISA method with human colonic cancer spectific monoclonal antibodes SC6 and SC3A. Forty -seven colorectal carcinomas, 33 colorectal adenomas, 15 hyperplastic polyps, 14 inflammatory polyps and 60 normal subjects were analysed. RESULTS The positivity rates of cancer associated antigen in serum and feces in colorectal cancers were 66.0% and 72.3% respectively, and in adenomas, 63.3% and 60.6%, hyperplastic polyps, 35.7% and 57. 1%, inflammatory polyps, 40. 0% and 33. 3%. But in normal group, the positivity rates were only 11.7% and 13.3%. The relation of Duke's stages of 45 carcinomas and the positivity rates of cancer associated antigen in serum and feces were further studied. The positivity rates for early colorectal cancers were 72.2% in feces and 50.0% in serum.CONCLUSION The detection of cancer associated antigens by ELISA method may be usefui for colorectal cancer diagnosis, and fecal detection may be more sensitive.