冠心病人血清中抗血管内皮细胞抗体水平的观察

Observation of Anti-Vascular Endotbeltal Cell(VEC) Antibody in Serum of Coronary Heart Disease Patients

本研究应用酶联免疫吸附试验（ＥＬＩＳＡ）夹心法，以人脐静脉内皮细胞为抗原，检测２６名冠心病人血清中抗血管内皮细胞抗体的水平，同时以２５名正常人作对照。结果表明，冠心病人血清中抗血管内皮细胞抗体水平显著高于正常人组（Ｐ＜０．００１）；抗体水平的高低与临床心电图的改变有一定相应关系。提示冠心病人体内有异常的免疫应答存在，抗血管内皮细胞抗体可能是冠心病的免疫学发病机理内容的一部分。临床对该病的防治工作中，除考虑脂质代谢紊乱的纠正外，也应注意对病人免疫功能的调整。

Serum level of anti-vascular endothelial cell (VEC) antibody was observed in 26 coronary heart disease patients and 25 age-matched normal persons. Cultured human umbilical vein endothelial cells were coated in the 96 well micro plate as antigen in this micro plate. Serum anti-VEC antibody was detected by using Sandwich ELISA method. The result showed that the anti-VEC auto antibody level in patients' serum is basically in parallel with the changes of their electrocardiographs. It is suggested that an autoimmune mechanism may play a role in the development of coronary heart disease, and the immunological regulation therapy is suggested for the patients besides the regular clinical treatment.