降钙素基因相关肽拮抗垂体后叶素引起心肌缺血性电生理改变的观察

The Observation of Antagonistic Effect of Calcitonin Gene-Related Peptide on the Changes of Ischemic Myocardial Electrophysiology Induced by Pituitrin

用浮置微电极技术观察垂体后叶素（Ｐｉｔ）对兔心肌细胞的异常电生理学效应及降钙素基因相关肽（ＣＧＲＰ）的保护作用。结果表明：静脉注射Ｐｉｔ后，心率减慢，心外膜电图ＳＴ段抬高，Ｔ波倒置，心室肌细胞跨膜动作电位（ＴＭＰ）的静息电位（ＲＰ）及动作电位振幅（ＡＰＡ）明显降低（Ｐ均＜０．０１）。复极化至３０％、５０％和９０％的动作电位时程（ＡＰＤ３０、ＡＰＤ５０、ＡＰＤ９０）亦明显缩短（Ｐ均＜０．０５）。静脉注射Ｐｉｔ同时注射ＣＧＲＰ，可见ＣＧＲＰ明显拮抗以上异常改变，从而起到保护心肌和稳定心肌的电生理作用。

The abnormal myocardial electrophysiological changes of Pituitrin and protective effects of Calcitonin gene-related peptide(CGRP)were observed with floating microelectrode technique in rabbit heart.After Pituitrin administered intravenously, heart rate(HR)slowed,ST segment of epicardial electrogram(EEG) elevated with a reverse of T wave and resting potential(RP),action potential amplitude (APA)decreased obviously(P<0.01),also the action potential duration(APD30,APD50,APD90) shortened significantly(P<0.05).But with the simultaneous administration of Pituitrin and CGRP,the above abnomal changes were obviously alleviated.These results indicated that Putuitrin could induce the changes of ischemic myocardial electrophysiology and CGRP might develop the stabilizing and protective effects on the ischemic myocardial electrophysiology.