摘要
应用Southern印迹分子杂交和免疫组化技术,检测18例肾穿刺组织中HBVDNA和HBV相关抗原(HBsAg、HBcAg和HBeAg)的阳性情况。结果表明15例HBVDNA阳性,其中8例成人和4例儿童为整合型,另3例儿童为游离型;有13例HBV抗原阳性。除肾小球外,肾小管亦麦达HBV抗原,提示HBVDNA的存在状态在成人主要为整合型,在儿童有整合型和游离型。讨论了HBVDNA的存在与HBV抗原表达的关系。
HBV DNA in renal biopsy specimens from 18 glomerulonephritis patients were detected by southern blot hybridization,with the HBV antigens in them examined simultaneously by immunohistochemical techniques.It was found that HBV DNA in the renal tissue were positive in 15 cases and appeared in 2 forms,i.e.integrated HBV DNA in 8 adults and 4 children and non-replicative free HBV DNA in the other 3 children.HBV antigens were found in 13 of the 15 HBV DNA positive renal tissue specimens.It can be seen that the HBV DNA in renal tissue was mainly of integrated form in adults and was of either free or integrated form in children. The relationships between the existence of HBV DNA in renal tissue and the expression of HBV markers in infected renal tissue and blood serum are discussed. The significance of HBV DNA and HBV markers in renal tissue in the diagnosis of HBV-related nephritis is defined.
出处
《徐州医学院学报》
CAS
1995年第4期360-363,共4页
Acta Academiae Medicinae Xuzhou
基金
国家自然科学基金
关键词
肾炎
乙型肝炎病毒
免疫组织化学
脱氧核糖核酸
glomerulonephritis
hepatitis B virus antigens
hybridization
immunohistochemistry