草原革蜱各发育阶段对驽巴贝斯虫传播能力的研究

RESEARCH ON THE ABILITY OF VARIOUS DEVELOPMENT STAGES OF DERMACENTOR NUTTALLI TO TRANSMIT BABESIA CABALLI

将实验室培育好的“清洁”草原革蜱成虫，释放到驽巴贝斯虫单一种人工感染的驴体上，使其自行叮咬吸血，俟雌虫饱血脱落后，置２８℃、相对湿度约９０％的温箱中产卵孵化。而后用次代３个不同发育阶段的蜱，分别叮咬除脾或非除脾健康易感驴。试验结果表明，草原革蜱在成虫阶段被驽巴贝斯虫感染，并经卵传递。次代幼虫、若虫和成虫都具有传播该种病原的能力。另外，还发现在草原革蝉卵内发育的驾巴贝斯虫，对易感宿主动物也具有感染性。

The“clean”adult Dermacentor nuttalli cultivated in the lab are allowed to hite andtake blood from the donkey infected by the single species of Babesia caballi.When thefemale Dermacentor nuttalli have taken enough blood and taken off the donkey′s body,they are put into the incubator to oviposit and incubate with the temperature at 28℃and R.H. at 90%,Then the Dermacentor nuttalli at three different stages of developmentare allowed to bite the splenectomized donkey and the unsplenectomized donkey respecti-vely.The experin1ent sholvs that Dermacentor nuttalli transmits Babesia cabalii to donkeyby transovarian transmission.The second-generation larvae, nyznphs and adults are allcapable of transmitting the pathogeny.It has also been discovered that the Babesia cabailideveloped in eggs of Dermacentor nuttalli are capahle of infecting the host animal.