摘要
应用５种限制性核酸内切酶（ＢａｍＨＩ、ＫｐｎＩ，ＳａｌＩ、ＢｇｌⅡ和ＨｉｎｄⅢ）建立了伪狂犬病毒闽Ａ株ＤＮＡ的内切酶图谱，并与Ｂｕｋ株、Ｓｈｏｐｅ株、Ｎｏｒｄｅｎ株、Ｓ株，台湾ＮＴＵ株进行比较，认为闽Ａ株与美国或欧洲毒株无关，而与台湾株同源，表明核酸限制性内切酶分析在伪狂犬病病毒研究中是一种毒株鉴别、分子流行病学调查的有用工具。
The DNA of pseudorabies virus Min-A strain was digested with five restriction end-onuclease of BamH I, Kpn I, Sal I, Bgl Ⅱ:, Hind Ⅲ, the size of fragments werecalculated and compared with those of Buk, Shope, Norden as well as Taiwan's NTUstrain. The results showed that Min-A strain had no homogenity with America strains andRomania strain and shared homogenity with Taiwan strain. The test indicated the restri-ction endonuclease analysis was a useful tool in differentiating Pseudorabies virus strainand epidemical survey.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1995年第2期134-138,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
