家兔微注射基因胚胎自体移植和异体移植的比较试验

COMPARISION BETWEEN AUTOGRAFTING AND ISOGRAFT RABBIT OVA MICRINJECTED WITH FOREIGN GENE

冲取３２只超排或自然发情配种的母兔早期胚胎４２７枚，选其中导入抗猪瘟Ｒｉｂｏｚｙｍｅ基因后的３４７枚优质胚胎分组移植不同受体。第一组将９３枚微注射卵移植到７只冲卵后的洪体输卵管内，６只妊娠并产仔３２只。经ＰＣＲ检测３２只仔兔中有２只整合了外源基因。第二组将２５４枚微注射卵移植异体受体１３只，妊娠６只并产仔２０只。经ＰＣＲ检测２０只仔兔中有２只整合了外源基因。

The advantage of autografting of rabbit ova microinjected with Ribozyme gene fordisease resistant was studied.There were 347 injected ova to be transferred into twogroup recipients. In the autografting group,a total of 93 injected elnbryos were tran-sferred into seven recipients,six of which become pregnant and produced 32 of fsprings, detection of the kittens using PCR confirmed that two of、which integrated foreignDNA. In the isograft group, There were 254 injected ova to be transferred into thirteenrecipients,six of which became pregnant and produced 20 offsprings,detection of thekittens using PCR confirmed that two of which contained the injected DNA.The resultdemonstrated that autografting gives higher the pregnancy rate and the embryo surviverate than isograft.