唾液获得性膜原位葡聚糖与变形链球菌和茸毛链球菌附着关系的实验研究

The effects of glucaus synthesized in situ iu experimental salivary pellicle on adherence of streptococcus mutans and streptococcus sobrinus.

通过离体实验观察了实验性获得性膜原位葡聚糖在变形链球菌Ｓ．ｍｕｔａｎｓ和Ｓ．ｓｏｂｒｉｎｕｓ附着牙面过程中的作用。结果表明：两菌具有相似的产生ＧＴＦ和原位葡聚糖的能力，但两菌附着量和附着率的差别较大。在有蔗糖和ＧＴＦ存在时，Ｓ，ｓｏｂｒｉｎｕｓ的附着率明显高于Ｓ．ｍｕｔａｎｓ．提示原位葡聚糖在Ｓ．ｓｏｂｒｉｎｕｓ的附着中起重要作用，Ｓ、ｓｏｂｒｉｎｕｓ主要以蔗糖依赖性机制定居牙面，而Ｓ．ｍｕｔａｎｓ主要以蔗糖非依赖性机制定居牙面。

The commonly used hydroxyapatite adherence assay was modified to include the incorporation of glucosyltransferaese and the synthesis of glucans in situ on saliva-coated hydroxyapatite beads. The laboratory strains of S. mutans and S. sobrinus were examined for their ability to adherence to experimental pellicles, either with or without the prior formation of glucans in situ. The results showed that both strains were able to produce GTF synthesing in situ glucan. However, there was evidently difference in the amount or rate of adherence between the two.In the presence of sucrose and GTF, the adherent rate of S. sobrinus was higher than that of S.mutans.It is suggested that glucans synthesized in situ is more important in adherence of S. sobrinus.