牙龈卟啉菌核酸探针在临床牙周炎检测中的应用

Application of Pg nucleic acid probe in clinical examination of periodontitis

随机选择门诊牙周炎患者１００人；无口腔感染性疾病者１００人。采用无菌纸捻从牙周袋（或龈沟）内吸取标本，用核酸探针杂交检测。结果表明：牙周炎中Ｐｇ的阳性率为８１％，而正常对照组中Ｐｇ阳性率仅为２８％，两组之间Ｐｇ阳性率相差非常显著（Ｐ〈０．０１０；有序分组资料的线性趋势检验分析显示：牙周炎中Ｐｇ随牙齿松动度的增大、牙周袋的加深、患者年龄的增高呈线性递增趋势（Ｐ〈０．０１）。

periodontitis patients and 100 subjects without oral infectious diseases were randomly chosen.By sterile paper points,samples were extracted from periodontal pocket(or gingival sulcus).Samples were checked by probe hybridization.As was shown by statistical analysis,1) Pg positive rate of periodontitis samples was 81% whereas that of health control group was only 28%.Difference between Pg positive rates of periodontitis group and healthy control group was of great significance(p<0.01),Test of linear trend showed that Pg of periodontitis patients showed linear crescendo tendency(P<0.01) with the increase in tooth mobility, depth of periodontal pocket and the age of the patients.The results indicate that Pg is closely related with periodontitis and plays an important role in tissue destruction.Nucleic acid probes promise a considerable prospect for clinical application.