麦角固醇高产率酵母菌株的筛选及其培养条件的研究

Selection of A High Ergosterol-producing Yeast and StUdies on Its Culture Conditions

从实验室保存的２０株酵母菌中筛选出麦角固醇含量较高的优良菌株奇异酵母２００５。以糖蜜为主要原料，采用正交试验方法考查了培养条件对奇异酵母２００５麦角固醇产量的影响。磷酸盐浓度的影响极显著（ａ＝０．０１），以不添加磷酸盐为最好。氦源种类也有显著影响（ａ＝０．０５），０．２４％氯化镀为氮源时产量最高。在优化条件下，麦角固醇产率可达到３．４２％（对细胞干重）。

A high ergosterol-producing strain.Saccharomyces Paradoxus 2005,is selected from 20yeasts in our laboratory. Influences of culture conditions on ergosterol production of S.aradoxus2005 are studied through orthogonal experiments using molasses media. Phosphate concentrationshows great significant influence(a=0.01)and it is most suited not to add phosphate to media。Kind of nitrogen source shows significant influence(a=0.05)and the best nitrogen source is0. 24%ammonium chloride. Under the optimum conditions,ergosterol yield can reach 3.42%ofcell dry weight.