黑熊血清蛋白与磺胺对甲氧嘧啶的结合率测定

Determination on Black Bear Serum Protein Binding Rate to Sulfamethoxydiazinum

本文用平衡透析法测定了黑熊血清蛋白与磺胺对甲氧嘧啶（ＳＭＤ）的结合率（ｆｂ）为４１．７７±１１．６６（５．３８～２５．８７）％．数据经Ｗｏｏｌｆ作图法处理、直线回归后，得到结合参数βｐ和Ｋｄｐ分别为：８．８５±１．７８μＭ／ｇ，６４７．２０±２４２．８４μＭ／Ｌ．黑熊的ｆｂ均随药物浓度的增高而降低，高浓度的呈现饱和现象．不同浓度间的ｆｂ差异极显著（Ｐ＜０．０１），不同个体间的ｆｂ差异极显著（Ｐ＜０．０１）．

With equilibrium dialysis method, it was detemined that black bear serum protein binding rate tO SMD was 41.77±11.66 (58.38～25.87)%. After the data treated with woolf plot, linear regressed, binding parmeter βp and Kdp were 8.85±1.78μM/g, 647. 20±242. 84μM/L respesively. Black bar fo was reducing with the medicine concentration increasing. High concentration medicine presented saturable phenomena. The difference of from different concentrations was very significant (P<0.01), So does it from different individual Pp<0.01).