PCR扩增抗乙酰胆碱受体单克隆抗体重链可变区基因的反应条件

Amplification of the heavy chain variable region gene of a monoclonal antibody against acetylcholine receptor by polymerase chain reaction

应用多聚酶链反应（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＰＣＲ）扩增抗乙酰胆碱受体单抗重链可变区的基因，探讨了ＰＣＲ的反应条件和影响因素。

Polymerase chain reaction(PCR)was used to amplify the heavy chain variable region gene of a monoclonal antibody against acetylcholine receptor.The reaction conditions and affect factors on PCR were investigated.