局部应用氧氟沙星脂质体治疗兔实验性绿脓杆菌性角结膜炎的研究

Investigation of topical ofloxacin liposomes in the treatment of experimental pseudomonas keratoconjunctivitis in rabbits

兔角膜实质接种绿脓杆菌,制成绿脓杆菌性角结膜炎模型。空脂质体为对照组,氧氟沙星脂质体和氧氟沙星滴眼剂为实验组。治疗6次后,观察结膜和角膜病变,井收集结膜囊分泌物进行培养。结果:结膜病变,第6、8、11天,两实验组与对照组相比都有显著性差异,第6天,氧氟沙星脂质体组明显好于氧氟沙星滴眼剂组。角膜病变,第6、8、11天,氧氟沙星脂质体组好于对照组,有显著性差异,第11天,氧氟沙星滴眼剂组与对照组相比有显著性差异。细菌培养结果,第6天,两实验组与对照组相比,有显著性差异,第11天,氧氟沙星脂质体组和氧氟沙星滴眼剂组的培养阳性率都增加了,但氧氟沙星滴眼剂组阳性率的增加程度高于氧氟沙星脂质体组。结论:氧氟沙星脂质体和氧氟沙星滴眼剂都可用来治疗绿脓杆菌性角结膜炎,氧氟沙星脂质体的作用优于氧氟沙星滴眼剂。

Pseudomonas aeruginosa was inoculated into corneal stroma of rabbits and the model of keratoconjunc tivitis was established. Empty liposomes were used in a control, group ofloxacin liposome and ofloxacin eyedrop groups as experimental ones. After treatment for six times conjunctival and corneal lesions were examinated. Swabs of conjunctival sac were cultured. Result: in conjunctiva, lesions in the two experimental groups were all significantly less than those in the control group on the 6th, 8th and 11th day. Lesions in the ofloxacin liposomes group were also significantly less than those in the ofloxacin eyedrop group on the6th day. In cornea, lesions in the ofloxacin liposomes group were significantly less than those in the control group on the 6 th, 8th and 11th day. Lesions in the ofloxacin eyedrop group were significantly less than those the control group on the 11th day. In the result of bacterium culture, the culture's positive rate of two experimental gronqs and control group had significant differences on the 6th day. On the 11th day, the positive rate the ofloxacin liposome groups and ofloxacin eyedrop groups increased, and in the ofloxaein liposomes group had a less increase. Conclusion: ofloxacin liposomes and ofloxacin eyedrops were effective in treatments of pseudomonas keratoconjuntivitis in the rabbit model, the effects of ofloxacin liposomes were better than that of ofloxacin eyedrops.