α_2-巨球蛋白治疗角膜硷烧伤的实验研究

Experimental study of treating corneal alkali burns with α_2-macroglobulin

用从家兔血浆中提纯的α_2-巨球蛋白(α_2-macroglobin α_2M)对实验性兔角膜硷烧伤进行了治疗,目的在于寻找一种新的快速、有效治疗眼部硷烧伤的新方法,并试图从体内找出一种新的清除自由基物质。首先从正常家兔血浆中提取制得纯度很高的α_2M滴眼液。在硷烧伤后第1、2、3、4和7天分别对兔眼作了外眼观察;在第2、7和14天分别抽取房水,对房水总SOD含量做了测定;并在第7和14天测定了房水总蛋白。结果表明,在烧伤后的第1,2和3天实验组家兔眼混合充血明显低于对照组,在第2和3天实验组角膜混浊水肿也明显低于对照组,统计学均有显著性差异。在第2、7和14天房水总SOD均高于对照组,统计学也有显著性差异,但两次测得的房水总蛋白含量均无统计学意义,但实验组蛋白含量均较对照组低。结论:α_2M对免眼硷烧伤的急性期炎症具有明显的抑制作用,对硷烧伤后组织产生的超氧自由基及其代谢产物具有清除作用。

The study aimed to search for a new, rapid and effective treatment of ocular alkali burns,and a new free radical scavenging agent. Experimental corneal alkali burns in rabbits were treated with highly purified α_2-maeroglobulin drops extracted from rabbit plasma. On the 1st, 2nd, 3rd, 4th, and 7th day after the burn, examinations of the outer eye were done, On the 2nd, 7th, and 14th day, samples of aqueous humor were drawn to test for theirtotal SOD content. On the 7th and the 14th day the total aqueous protein was also determined. The results showed that on the 1st, 2nd, and 3rd day after the burn, mixed congestion in the experimental group animals was markedly milder than that in the the control group, and on the 2nd and 3rd day, opacity and swelling of the cornea were also less severe in the experimental group. On the 2nd, 7th, and 14th day the tot al aqueous SOD contents of the experimental group were all higher than those of the eontrol samples, but the total aqueous protein which was determined twice showed no statistical significance in the two groups of animals, It was concluded that α_2-macroglohulin had a marked inhibitory effect on the acute inflammatory reaction of the rabbit corne of alkali burns, and that it also had the power of doing away with superoxide free radieals in the damaged tissue and their metabolites.