HPLC法测定丹皮中丹皮酚的含量

DETERMINATION OF PAEONOL IN THE CHINESE TRADITIONAL DRUG CORTEX MOUTAN RADICIS (PAEONIA SUFFRUTICOSA ANDR.) BY HPLC

牡丹皮为毛茛科牡丹Paeonia suffruticosa Andr.的根皮,常用作清热、凉血药,丹皮酚是其主要成分之一,具有降压、止血等多种药理作用。丹皮酚含量测定方法有直接蒸馏-UV法、HPLC法等。

A reversed-phase high performance liquid chromatographic method for determination of paeonol contained in Cortex Moutan Radicis was proposed. Paeonol contained in Cortex Moutan Radicis and internal standard, phenacetin, was extracted with methanol with the aid of ultrasonic cleaner and the extract was directly analyzed on NOVA-PAK C_(18)(150×3.9mm, 5μm) using CH_3OH·H_2O(1:1) as the mobile phase.Calibration graph was linear in the range of 0.06～0.84μg and recovery was 99.92 % (CV 2.2 %, n=10). The paeonol contents in P.Suffruticosa from diffrent territories were detected and were agreeable with those obtained by direct steam distillation-UV method.