高效液相色谱法测定人血浆中尼莫地平浓度

HPLC Determination of Nimodipine in Human Plasma

本文以反相高效液相色谱法测定人血浆中尼莫地平的浓度。以尼群地平和甲基睾丸素为内标物，碱性条件下用乙醚－正已烷（１：１）萃取，流动相为甲醇－水－正丁胺（６５：３５：０，００１），流速１．２ｍｌ／ｍｉｎ，紫外检测波长２３８ｎｍ，线性范围５～１００ｎｇ／ｍｌ，最低检测浓度２ｎｇ／ｍｌ，日内差异小于５％，日间差异约为５％。６名健康受试者口服－种国产尼莫地平片剂后，以本方法测定其体内药代动力学符合一室模型。

A HPLC method for the determination of nimodipine in human plasma was described.Under alkaline condition,nimodipine and internal standards nitrendipine and methyltestosterone are extracted with mixed solvent of ether and n-hexane(1:1).Then the separation is performed in an ODS column with a mobile phase of methanol-water-n-butylamine(65: 35 :0.001)and detected at 238nm.The linear concentration range of this method is 5～100 ng/ml and the detectable lowest concentration is 2ng/ml (S/N=3).This method has been applied to the determination of pharmacokinetic parameters of six normal volunteers after single dose administration of nimodipine tablets. Results show that the pharmacokinetic parameters fit with one compartment model.