光纤化学传感器—流动注射分析联用技术在线监测兔尿中呋喃妥因浓度

ON-LINE MONITORING OF NITROFURANTOIN IN URINE OF RABBIT BY A FLOW-INJECTION SYSTEM WITH A FIBER-OPTIC CHEMICAL SENSOR

用光纤化学传感器－流动注射分析联用技术，以固定了荧光分子探针芘丁酸的醋酸纤维素膜作为分析物识别器，在线监测兔尿中呋喃妥因浓度。方法的回收率为９６．５％～１０２．９％。日内、日间ＲＳＤ为１．５％～３．８％。尿药浓度在２～１００μｇ·ｍｌ－１范围内呈线性关系，相关系数０．９９８３。当信噪比为３时，最低检出限为０．７４μｇ·ｍｌ－１。应用该法测定了６只家兔口服呋喃妥因后的尿药一时间曲线，并据此提取了药代动力学参数。同时用高效薄层色谱法对该法的选择性进行了验证。

A flow-injection system for monitoring of nitrofurantoin(NFT) in rabbit urinethat makes use of a kind of fiber-optic chemical sensor is presented. The NFT-sensitive optrode wascombined with a membrane onto which was immobilized pyrenebutyric acid(PBA)as a fluoroprobe.The NFT concentration was determined via dynamic quenching of the fluorescence of PBA. Theaverage recovery was 96. 5%～102.9%. The relative standard deviations for within-day andbetween-day were 3.1/～3.7%and 1.6%～3.8%respectively. The calibration curve was linearin the range from 2μg·ml-1 to 100 μ g·ml-1 with r=0.9983. The detection limit of this method was0.74μ g·ml-1. The urine drug concentration-time course in rabbit after oral administrationconformed to a one compartment open model with a first order absorption phase. Mean T1/2 value was0.75±0. 14h. The selectivity was affirmed by HPTLC.