蒸发光散射检测器测定脂肪乳注射液中甘油三酯含量的研究

Study on Determination of the Content of Triglycerides in Fat Emulsions using ELSD

本文建立了高效液相色谱法分离、蒸发光散射检测器测定脂肪乳注射液中甘油三酯含量的方法。在硅胶柱上以正己烷－异丙醇－乙酸（９８．９：１：０．１）为流动相，测得甘油三酯的回收率为９９．８９±０．５９％，ＲＳＤ为０．７４％（ｎ＝８），进样量在４～１６μｇ时，线性方程：Ｙ＝－２．４５×１０￣５＋９．１５×１０￣５Ｘ，ｒ＝＝０．９９６５。

A HPLC method for the determination of the content of Triglycerides in fat emulsions using ELSD has been developed.The porous silica particles column and n-hexane/iso-propyl alcohol/acetic acid (98.9:1:0.1) as mobile phase were used. The recovery of triglycerides is 99.89±0.59%The RSD are 0.74%(n=8).The linear range is from 4 to 16μg.The regression equation is Y=2.45×105+9.15×105X,and the correlation is good with r=0.9965.