重组人生长激素中宿主蛋白的点─免疫结合测定法

Dot─immunobinding Technique for the Detection of E. coli Proteins inRecombinant DNA Derived Human Growth Hormone

本文应用碱性磷酸酶免疫斑点法，检测重组人生长激素中Ｅ．ｃｏｌｉ蛋白质。该法的最低检出量为１．９ｎｇ；线性范围为２．５～０．０３９μｇ／ｍｌ；平均回收率为１１５．２％，ＲＳＤ＝６．９９％（ｎ＝８）。并具有灵敏、特异、操作简便等特点。

A dot-immunobinding technique on nitrocellose membranes has been used for the quantification and semi -quantification of E.coli proteins in r-hGH. Alkaline phosphatase was applied to this experiment. The lowest limits of detection was 1.9ng. Recovery was 115%(RSD=6.99%).A linear relation is obtained in the range of 0.5～0.039μg/ml. The results for sample detection are satisfactory.This method is sensitive,reporducible rapid and simple. This technique provide a comprehensive approach to quality control of rDNA Pharmaceuticals.