RP－HPLC法测定人血清中卡马西平及其环氧化物

Simultaneous Determination of Carbamazepine and Its Epoxide Metabolite in Serum by HPLC

用ＲＰ－ＨＰＬＣ法测定人血清中卡马西平（ＣＢＺ）及活性代谢产物卡马西平１０，１１－环氧化物（ＣＢＺ－Ｅ）的含量。血清样品用乙醚－二氯甲烷（３：１，Ｖ／Ｖ）混合溶剂提取，以硝基安定为内标，乙腈－水（４０：６０，Ｖ／Ｖ）为流动相，流速为１．２０ｍｌ／ｍｉｎ，ＵＶ２１５ｎｍ处测定；ＣＢＺ－Ｅ和ＣＢＺ最低检测浓度分别为５０和６０ｎｇ／ｍｌ；线性范围为０．１～５．０μｇ／ｍｌ和０．２～２０μｇ／ｍｌ；ＲＳＤ在５％以下。应用本法测定了８名癫痫病人单一服用ＣＢＺ治疗血清中ＣＢＺ和ＣＢＺ－Ｅ的浓度。

A RP-HPLC method for the simultaneous determination of carbamazepine(CBZ)and carbamazepine-10,11-epoxide(CBZ-E)in serum was described. CBZ, CBZ-E and internal standard (IN. nitrazepam)were extracted by ether:dichloromethane(3:1,V/V).Separation of CBZ and CBZ-E was achieved by reversed-phase chromatography,using acetonitrile-water(40/60,V/V)as the mobile phase at a flow-rate of 1.20ml/min on a 5μm C18ODS colum. The retention time for CBZ-E, CBZ and IN were 3.1,4. 7 and 5.6 min,respectively.The detection limit CBZE and CBZ were 50 and 60ng/ml.The calibration curve was linear in the range from 0.1 to 5.0μg/ml(CBZ-E)and from 0.2 to 20.0μg/ml(CBZ).RSD was less than 5%. The method have been used to determine steady-state concentration of CBZ and CBZ-E in 8 epileptic patients receving monotherapy.