反相高效液相色谱法测定阿霉素的血药浓度

Determination of Concentration of ADM in Blood by RP-HPLC

本文采用反相ＨＰＬＣ法测定阿霉素的血药浓度，血浆样品用甲醇－氯仿（１：４）在ｐＨ９．０的条件下提取后进样，５ｍｍｏｌ／Ｌ磷酸－异丙醇－甲醇－乙腈（４５：３５：１０：１０，ｐＨ２．９）为流动相，荧光检测器λ＿（ｅｘ）＝４５０ｎｍ，λ＿（ｅｍ）＝５３０ｍｍ。最低检测浓度１０ｍｇ／ｍｌ，线性范围在３０～１５００ｎｇ／ｍｌ内ｒ＝０．９９８７，日内ＲＳＤ为１．５％～２．４％，日间ＲＳＤ为１．８％～３．７％。并对７例肺癌病例经支气管动脉灌注阿霉素的药代动力学进行了研究。

A RP-HPLC method for the determination of adriamycin in human plasma was reported.The ADM was extracted from 1ml of plasma at pH 9.0 by methanol-Trichloromethane(1:4).A solution of 5mmol/L orthophosphoric acid-propan-2-ol-methanol-acetonitrile(45:35:10:10)(pH2.9)was used as the mobile phase.The spectrofluorimeter for peaks detection with λ_(ex) =450nm,λ_(em)=530nm was used.The detection limit was 10ng/ml,the calibration curve was linear in the range from 30ng/ml to 1500ng/ml with r=0.9987.The RSD within a day were 1.5%～2.4%,and the RSD between days were 1.8%～3.7%.The method has been applied to study the pharmacokinetics of the adriamycin with intra-arterial infusion for 7 case of lung carcinoma.