摘要
本文以２－萘磺酸钠为内标，建立了ＲＰ－ＨＰＬＣ法测定兔血浆中安乃近及其３种活性代谢物ＦＡＡ、ＡＡ、ＭＡＡ的浓度。Ｓｈｉｍ－ＰａｃｋＣＬＣ－ＯＤＳ（１５ｃｍ×６ｍｍＩＤ）为分析柱；ＯＤＳ预柱；流动相组成：甲醇－水－０．５ｍｏｌ／Ｌ磷酸二氢钠－三乙胺（３５：６３：２：０．０１），磷酸调节ｐＨ６．０±０．５；流速：１．０ｍｌ／ｍｉｎ；柱温：３５℃；检测彼长：２６０ｎｍ。本法简便、快速、准确、灵敏，适用于安乃近及其活性代谢物的血药浓度测定及药物动力学研究。
A simple,specific and sensitive RP-HPLC analysis,using sodiumβ-naphthalene sulfonate as internal standard,was developed to simultaneously determine analginum and its main metabolites(FAA,AA,MAA)in rabbit plasma,Shim-pack CLC-ODS(15cm×6mmID)was used as analytical column.The mobile phase consists of MeOH-H2O-0.5mol/LNaH2PO4-N(C2H5)3 at 260nm.The column temperature was 35℃.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1995年第5期10-12,共3页
Chinese Journal of Pharmaceutical Analysis
