二阶导数光谱法测定尿中呋喃妥因浓度

Determination of nitrofurantoin in urine by second derivative spectrophotometry

本文采用有酸性条件下用乙酸乙酯萃取呋喃妥因后，通过二阶导数光谱法测定尿中呋喃妥因的浓度。该法定量信息为３７４～３７７ｎｍ处的峰谷之间距离，最低检出浓度为１．５ｍｇ／Ｌ，回收率为９８％，相对标准差小于３％；本法与ＨＰＬＣ法进行了对照性研究。两法具有明显的相关性（ｒ＝０．９９９１）。本法可用于呋喃妥因药动学的研究．

The paper teported the determination of nitrofuratoin in urine by second derivative spectroscopy. The method consists of direct extraction of the drug by the use of ethyl acetate from the acidic urine and determination of the nitrofurantoin concerntration by measuring the amplitude between the maximum and minimum of the second derivative signal at 374-377nm. The method had a detection limit of 1. 5mg/L with recovery of 98% and RSD was less than 3%. The both methods of second derivaive and HPLC had notable relativity.The method could apply to the pharmacokinetics study of nitorfurantoin.