摘要
用膜片钳法，在培养的Ｗｉｓｔａｒ大鼠心室肌细胞上记录了Ｌ型钙通道的单通道活动。乌头碱５０μｇ·ｍｌ－１能显著阻断Ｌ型钙通道的活动，使其开放时间缩短、关闭时间延长、开放概率下降。与对照组比较，有显著性统计学差异（Ｐ＜０．０１）。而对通过Ｌ型钙通道的钡离于流幅值无明显影响。
Ventricular myocardiocytes from neonatal Wistar rats were
isolated and cultured.Aconitine,Ca2+ channel blocker verapamil or
Ca2+ channel activator BAY K8644 were added to thebath solution
separately. Using the cell-attached configuration of the patch clamp
technique ,the singlechannel activities of L type Ca2+ channel were
recorded before and after addition of all three drugs.The results
showed the blocking effect of aconitine (50μg· ml-1)on L type Ca2+
channels. Itsmechanism may be relevant to the decrease in both open
state probability and the mean open time ofCa2+ channel. The
difference was statistically significant compared with control
group(P<0.01).The amplitude of Ba2+ currents ,which flow through open
L type Ca2+ channel was unchanged.
出处
《药学学报》
CAS
CSCD
北大核心
1995年第3期168-171,共4页
Acta Pharmaceutica Sinica
