脑内注射白细胞介素－1β对淋巴结细胞应激免疫抑制因子生成的影响

EFFECT OF INTRACEREBROVENTRICULAR INJECTION OF IL-1β ON GENERATION OF IMMUNO-SUPPRESSIVE FACTOR IN LYMPHOCYTES IN STRESSED MICE

脑内注射白细胞介素－１β对淋巴结细胞应激免疫抑制因子生成的影响李怡凡，左永昌，邵黎，宋德懋，丁桂凤，范少光（北京医科大学生理学系，北京医科大学免疫学系北京１０００８３）我室以前的工作发现束缚应激后，动物血清内出现一类能抑制淋巴细胞转化的因子，进一步的...

our previous work showed that a suppressive factor( a protein with largemolecular weight in serum was induced by restraint stress in mice and rats,which suppressed Con Ainduced lymphocyte proliferation.It was also found that the generation of serum suppressive factorwas under control of the central nervous system.Our further study showed thatintracerebroventricular(icv )injection of interleukin 1 receptor antagonist(IL-1Ra)antagonised thegeneration of serum suppressive factor induced by restraint stress and icv injection of interleukin-1β(IL-1β)increased the generation of the suppressive factor.Our experiment also showed that the serumsuppressive factor induced by restraint stress was first made in lymph tissue and then released intoblood.The present work was designed to investigate the role of IL-1 in the brain in generation of thesuppressive factor in lymph node in mice.Icv injection of IL-1 β( 1 pg/mouse) was shown tosignificantly increase the generation of the suppressive factor in lymph node.Icv injection of IL-1Ra,however ,antagonised generation of the suppressive factor.In mice without restraint stress,both thesuppressive factor in serum and in lymph node were found to be induced in dose-dependent manner byicv injection of IL-1β.Taken together,these results suggest that IL-1β in brain played a veryimportant role in generation of the suppressive factor in lymph node.The positive correlation betweenthe suppressive action of lymph node and of serum added to the evidence that lymph tissue is probablythe source of the serum suppressive factor.