摘要
体外培养的小鼠Ｌ１２１０细胞被ＨＨ０７Ａ２μｇ·ｍｌ－１作用２４ｈ后，与对照组细胞相比，其细胞数不再增长，有丝分裂数及集落形成率下降，细胞形态及细胞周期动力学均发生一定的变化。且ＨＨ０７Ａ大剂量短期作用抑制Ｌｌ２１０细胞集落形成的效率高于低剂量持续作用。
The L1210 cell rapidly ceased to
proliferate and its mitotic index decreased markedly after being
exposed to HH07A 2μg·ml-1 during exponential growth phase. However,
34.6% of the cells were still able to form colonies in soft agar if
HH07A was removed after 24 h ofincubation( the colony-forming
efficiency for control cells was 63.7%)and the cell viabilityremained
at about 94%.The DNA contents in L1210 cells were measured with a
flow-cytometer.Theresults showed that the cell-cycle was blocked at
the S phase and the number of cells in G2+Mphasedecreased
significantly.The time response course for L1210 cells indicated that
the inhibitory effect of HH07A on L1210 clonogenic cells was slightly
time dependent.
出处
《药学学报》
CAS
CSCD
北大核心
1995年第7期491-494,共4页
Acta Pharmaceutica Sinica
关键词
抗癌药
海南粗榧
集落形成率
L1210细胞
Anticancer drug
Cephalotasus hainanensis Li
Colony-forming rate
Cell-cycle
