用核磁共振新技术测定日本续断皂甙E＿1的结构

APPLICATION OF FNEW TECHNIQUES OF NMR IN THE STRUCTURE ELUCIDATION OF JAPONDIPSAPONIN E1ISOLATED FROM DIPSACUS J APONICUS MIq.

从日本续断ＤｉｐｓａｃｕｓＪａｐｏｎｉｃｕｓＭｉｑ．根的乙醇提取物中得到一个新三萜皂甙（５糖甙），命名为日本续断皂甙Ｅ１（ｊａｐｏｎｄｉｐｓａｐｏｎｉｎＥ１）。用化学方法及１ＨＮＭＲ，１３ＣＮＭＲ，１Ｈ－１ＨＣＯＳＹ，一维多重接力ＣＯＳＹ，选择性远程ＤＥＰＴ和三重共振ＮＯＥ差谱等方法，鉴定其结构为３－Ｏ－α－Ｌ－吡喃鼠李糖（１→３）－β－Ｄ－吡喃葡萄糖（１→３）－α－Ｌ－吡喃鼠李糖（１→２）－α－Ｌ－吡喃阿拉伯糖－常春藤皂甙元２８－Ｏ－β－Ｄ－吡喃葡萄糖甙。

A new triterpenoid saponin, named japondipsaponin E1(5 sugars ), was isolated from the ethanolic extract of the roots of Dipsacus japonicus Miq. The structure was elucidated by chemieal and spectral methods,e.g.1HNMR,13CNMR,1H-1HCOSY, ID multiple relay COSY,selectivc long range DEPT and NOEDS,to be 3-Oα-L-rhamnopyranosy1( l→3)-β -D-glucopyranosyl( 1→3)-α-L-rhamnopyranos1(1→2)-α -L-arabinopyranosyl-hederagenin 28-O-β-D-glucopyranoside.