关于E.coli中alk A基因启动子突变点的研究

STUDY OF ALK A PROMOTER MUTATION IN E. COLI

本文用人工突变的方法,获得E.coli.alk A基因启动子上游区的突变体,其中1个碱基置换的有73株,2个及2个以上碱基置换的有7株,缺失和插入的有11株。突变株β-半乳糖苷酶活性测定,观察到大部分酶活性低于野生型,仅1株高于野生型。

The upstream mutation of alk A gene promoter had been gained by artificial methcd. Among them there was 1 base substitution in 73 clones, there were 2 and more base substitution in 7 clones, and deletion and insertion in 11 clones. The β-galactosidasc activities of most mutant clones were lower than the wild type, and only one was higher than the wild type.