新维甲类化合物-R-81001对HL-60细胞的分化诱导作用

Induction of Differentiation of Human Promyelocytic Leukemia (HL-60) Cells by New Retinoid R-81001

应用软琼脂集落形成法选出单克隆HL-60细胞系,系统地研究新维甲类化合物R81001的分化诱导作用。证明该药可使人早幼粒白血病细胞的NBT还原能力明显增强,呈浓度依赖性。形态学观察表明,在药物作用下细胞的核浆比例降低,核仁减少或消失.胞浆中嗜天青颗粒减少,出现特异性颗粒。多数细胞处于中或晚幼粒细胞阶段。非特异酯酶染色试验证明,R81001诱导HL-60细胞沿粒系统定向分化。

By using soft agar colony-forming assay, a monoclonal subline of human promyelocytic leukemia HL-60 cells was isolated and inducing activity of differentiation of a new retinoid R-81001 on the cells was studied. Ph-enotypic changes, such as biologic behavior, morphological and biochemical characteristics, show that R-81001 can induce HL-60 cells to differentiate along the myloid pathway.