ND-O-BSA血清学活性及其在麻风血清诊断中的应用

Studies on the Serological Activity of ND-O-BSA and Its Application in Serodiagnosis of Leprosy

整麻风杆菌(Whole mycobacterium Leprae,WML)及其特异酚糖脂(Phenolic glycolipidI,PGI)血清学活性的研究报告不少,但因麻风杆菌(ML)与其它分枝杆菌间存在着广泛地交叉反应抗原,且PGI也与结核杆菌间有部分微弱的交叉反应,因而寻找新的更特异的抗原具有重要意义.ND-O-BSA是PGI特异部分的天然二糖(natural disacchride)与BSA(bovine serum al-bumin)相联结的半合成产物,理论上应比PGI特异。本文报告ND-O-BSA血清学活性及其与WML,PGI血清学活性的比较结果,并讨论三者的敏感性,特异性及在早期麻风诊断中实用意义。

In this article, optimum conditions of ND-O-BSA-ELISA were determined and comparisons made of the serologic activity -of 3 different types of antigens (WML, PGI, ND-O-BSA) in leprosy (151) and tuberculosis (20) patients and normal controls (42). The results indicated that: 1) optimum conditions for ND-0-BSA-ELISA were ND-O-BSA and OPD concentration of 0.1 and 400*g/ml respectively with HRP-IgM and testsera dilutions of 1 : 1000 and 1:200; and 2) serological activity showed highly significant positive correlations between 3 types of antigens (WML vs PGI: r = 0.88, P< 0.0005; PGI vs ND-O-BSA: r = 0.86, P<0.0005), all of which were highly sensitive and specific for leprosy serodiagnosis with positive rates of 100% (PGI) and 97.4% (WML, ND-O-BSA) in leprosy patients. None were positive in normal controls, but crossreactivity of different degree were present in tuberculosis patients. The authors suggest that PGI-ELISA and ND-O-BSA-ELISA be used in combination in the evaluation of clinical therapeutic efficacy and determination of disease stages, as well as in epidemiologic survey of subclinical leprosy infection in 'high-risk' areas.