小鼠骨髓红系祖细胞体外培养细胞分化的形态学观察

Morphological Observation of Erythroid Progenitor Cell Differentiation in vitro

用改良Iscove甲基纤维素法体外培养小鼠骨髓红系祖细胞,对形成的红系集落细胞分化进行形态学观察。结果发现:(1)在低浓度红细胞生成素培养条件下,处于较晚发育期的红系祖细胞(CFU-E)可形成数十个细胞组成的集落,但各集落间的细胞数量及发育阶段的同步性有较大差异;(2)在高浓度红细胞生成素培养条件下,处于较早发育期的红系祖细胞(BFU-E)可形成数千个甚至上万个细胞组成的大集落,且集落内细胞的发育阶段具较高的同步性;(3)在CFu-E和BFu-E培养集落中均可观察到自然出现的核固缩及排核现象。以上发现为哺乳类红细胞自然排核学说及红系终末细胞中存在不利于细胞核增殖生长的“胞质因子”工作假说提供了有利的佐证。

Iscove's methylcellulose method was adopted and modified for the culture of erythroid progenitor cells isolated from BABL/c mouse bone marrow, and in vitro differentiation of the erythroid cell colonies was observed morphologically.Experimental results indicated: (1) in culture medium with a low concentration of erythropoietin, colonies of different sizes composed of tens to several tens of largely unsynchronized cells were formed from the late erythroid progenitor cells (CFU-E): (2) in culture medium with a high concentration of erythropoietin, the early erythroid progenitor cells (BFU-E) could be stimulated to form larger colonies which consisted of several thousand to ten thousand more highly synchronized cells; and (3) the process of pyknosis and extrusion of nuclei from the late erythroblasts could be readily observed in both the CFU-E and BFU-E colonies.