利用α-factor启动子在酵母中表达乙型肝炎表面抗原基因

Expression of Gene Encoding HBsAg in Yeast Cell with Its α-factor Promoter

利用酵母α-factor分泌型启动子构建大肠杆菌—酵母菌穿梭质粒。将乙型肝炎表面抗原基因(HBsAg基因)经过调正读码框架使其准确地和表达载体相连接,最终在酵母中成功地表达HBsAg蛋白并分泌到细胞外。

Construction of an E. coli-yeast shuttle plasmid was reported with yeast α-factor as promoter of synthesis and secretion of mature foreign proteins by yeast cells (Saccharomyces cerevisiae). The HBsAg gene was sucessfully recombined into the plasmid with its frame precisely adjusted, thus resulting in extracellular secretion of HBsAg protein by the yeast cells.