摘要
作者用高效液相层析（ＨＰＬＣ）法测定了汉、回、维吾尔（维）、哈萨克（哈）族新生儿胎儿血红蛋白（ＨｂＦ）中~Ｇγ／~Ａγ、~Ａγ~Ｉ／~Ａγ~Ｔ比值，共３７２例，其中汉族和回族各１０２例、维族９９例、哈族６９例。％~Ｇγ（~Ｇγ／~Ａγ）均值：汉、回、维、哈族分别为６６．８３％、６８．３３％、７０．４４％和６９．７０％。低~Ｇγ者（＜５０％）４例：其中回族３例、哈族１例。高~Ｇγ者（＞８０％）２５例：汉和哈族各５例、回族４例，维族１１例。发现~Ａγ~Ｔ杂合子９９例分别为：汉２１例、回２３例、维２６例、哈族２９例。４个民族各发现１例~Ａγ~Ｔ纯合子。％~Ａγ~Ｉ（~Ａγ~Ｉ／~Ａγ~Ｔ）均值：汉５６．８３％、回５５．５８％、维５０．９４％、哈５４．６８％。~Ａγ~Ｔ基因频率依次为０．１１３、０．１２３、０．１４１及０．２２５。两例比值异常者（１例维族高~Ｇγ８５．４２％、~Ａγ~Ｔ杂合体。１例回族低~Ｇγ４３．４％，~Ａγ~Ｉ纯合体）经γ基因图谱分析，确定高~Ｇγ值者γ珠蛋白基因型为－~Ｇγ－^（ＡＧ）γ－~Ａγ~Ｔ（~Ａγ~Ｉ）－／－~Ｇγ－~Ａγ~Ｉ，（~Ａγ~Ｔ）－；低~Ｇγ值者为－^（ＧＡ）γ~Ｉ－／－~Ｇγ－~Ａγ~Ｉ－。
The Gγ/Aγ, AγI/AγT ratios of fetal hemoglobin of 372 cord blood samples of newborns(Han 102, Hui 102, Uygur 99,and Kazak 69)in Urumchi were determined by HPLC. The means of Gγ ratios of 4 ethnic groups(Han, Hui, Uygur, and Kazak)were 66.83%, 68.33%, 70.44% and 69.70%, respectively. 4 cases(Hui 3 and Kazak 1)with low Gγ(<50%),and 25 cases(Han 5,Hui 4,Uygur 11 and Kazak 5)with high Gγ(>80%) were found.The occurrences of low Gγ in Hui and Kazak were 2.94% and 1.45% while in Han and Uygur no single case with low Gγ was found. Those of high Gγ in 4 ethnic groups were 4.9%(Han), 3.9%(Hui),11.1%(Uygur) and 7.2%(Kazak), respectively. AγT heterozygotes in Han 21, Hui 23, Uygur 26 and Kazak 29 cases, and one AγT homozygote in each ethnic group were found. The average values of AγI were 56.83%, 55.58%, 50.94% and 54.68% respectively.The frequencies of AγT gene were 0.113,0.123,0.141 and 0.225, respectively.The gene arrangement of a low Gγ case was identified as-(GA)γI-/-Gγ-AγI-,and that of a high Gγ case as -Gγ-(AG)γ-AγI(AγI)-/-Gγ-AγT(AγI)-.Received February 26,1993.This project supported by National Natural Science foundation of china
基金
国家自然科学基金
