摘要
本实验用ＡｐａＩ、ＡｖａＩ、ＢａｍＨＩ、ＢｃｌＩ、ＢｇｌＩＩ、ＢｇｌＩＩ、ＣｌａＩ、ＥｃｏＲＩ、ＥｃｏＲＶ、ＨｉｎｄＩＩＩ、Ｈｐａｌ、ＫｐｎＩ、ＰｓｔＩ、ＰｖｕＩＩ、ＳａｃＩ、ＳａｌＩ、ＳｃａＩ、ＳｍａＩ、ＸｂａＩ和ＸｈｏＩ等２０种限制性内切酶分析来自中国西南地区的家猪和野猪的ｍｔＤＮＡ群体遗传多样性。结果表明：在全部２８只个体中，共检出２６种限制性态型（ｍｏｒｐｈ），归结为６种不同的限制性类型，限制性类型的差异主要来源于少数几个限制性位点的偶然突变。利用现代分子群体遗传学方法，对这些猪的遗传多样性进行评估，结果表明中国西南地区猪的ｍｔＤＮＡ变异度很低，遗传多样性贫乏，提示西南地区的猪起源于一个共同的祖先，在品种形成的早期可能受到创立者效应的制约。
Twenty restriction endonucleases, ApaI, AvaI, BamHI, BclI, BglI, BglII, ClaI,EcoRI, EcoRV,HindIII, Hpal, KpnI, Pstl, PvuII, SacI, SalI, ScaI, SmaI, XbaI,XhoI were used to investigate the mitochondrial DNA polymorphism of pigs from 11 localities in southwest China.Among the 28 animals analyzed, 27 restriction morphs were detected, which can be sorted into 6 haplotypes. The average genetic distance among haplotypes(P)is 0.004,and the value of average sequence variation in pig population(π) is 0.122%. Our results suggest that the genetic diversity within the pigs is remarkably scarce. The pigs in southwest China may have origineted from a late common ancester,and were likely impacted by the founder effect.
