摘要
通过种子半粒法，采用聚丙烯酰胺凝胶等电电泳和人工接种Ｍｅｌｏｉｄｏｇｙｎｅｎａａｓｉ抗性鉴定相结合，分析Ａｅ．ｖａｒｉａｂｉｌｉｓＮｏ．１×Ａｅ．ｖａｒｉａｂｉｌｉｓＮｏ．２Ｆ２植株酯酶Ｅｓｔ－５标记同功酶与抗性基因Ｒｋｎ－ｍｎｌ连锁程度，发现标记同功酶受染色体３Ｕ或３Ｓ~Ｖ长臂上１对共显性基因控制，与抗性基因连锁程度较高，重组率为１２．９６±０．４０％，图距为１３．２６±１０．４６分摩。这样，在抗性基因Ｒｋｎ－ｍｎｌ转移中，可利用标记同功酶大量、快速、高效鉴定植株抗线虫性。
Analyses of marker isozyme esterase Est-5 and resistance gene Rkn-mnl usingsemi-grain of F_2 plants of Ae. variabilis No.1×Ae. variabilis No.2 by polyacrylamide gel isoelectric focusing (PAG-IEF)and by artificial inoculation of M. naasishowed that the marker isozyme was encoded by a pair codominant alleles on thelong arm of chromosome 3U or 3S￣V,and it was linked with the resistance gene with a recombination value of 12.96±0.40%,genetic distance of 13.26±0.40cM. Therefore, the marker isozyme could be used for testing plant resistance to cereal rootknot nematode rapidly, efficiently and quantitively in transfer resistance gene Rknmnl.Received July 29,1993
