摘要
将由ＳＶ４０早期启动子和小鼠金属硫蛋白基因启动子所控制的人凝血Ⅸ因子ｃＤＮＡ的重组基因分别导入培养的小鼠成纤维细胞，发现它们都能被表达。进而将它们分别导入小鼠受精卵雄原核，作成相应的转基因小鼠，则发现它们都失去了表达特性，结果分析表明，在人凝血Ⅸ因子ｃＤＮＡ中可能存在着决定其在活体内表达的顺式调节元件。
Two recombinant genes containing human coagulation factor Ⅸ cDNA driven by SV40 early promoter or by mouse MT promoter were introducted into the cultured mouse fabroblasts,respectively.It was found that both of them could be expressed in the cultured cells.Then,the two recombinant genes were microinjected into the male pronuclei of fertillized mouse eggs for generating transgenic mice harbouring the introduced genes,respectively.However,we did not observed that the two genes could be expressed in transgenic mice.These results suggest that the in vivo expression of human coagulation factor Ⅸ cDNA be controlled by some cis regulatory element(s).
基金
国家高技术发展计划资助
