摘要
采用水平切片淀粉凝胶电泳技术，进行３０头独龙牛４１种蛋白质共计４４个遗传座位的等位酶分析，只在Ｔｆ、Ｈｐ、Ａｍｙ、Ｅｓｔ等４个座位发现多态性。每个座位等位基因的平均数、多态座位百分比和平均杂合度值分别为Ａ＝１．０９０９、Ｐ＝０．０６８２和Ｈ＝０．０２６２。贡山县和福贡县独龙牛群体从酶基因的角度上看遗传多样性贫乏，可能是分别由小种群引种而来，受到瓶颈效应的作用，并伴随着创立者事件的发生。我们结合独龙牛在自然条件下的生存情况，对独龙牛的繁殖和保护进行了遗传管理方面的初步探讨。
In the present study, analysis of 44 gene loci in the population Mithun(Bos frontalis)of 30 individuals were carried out by means of horisontal starch gel electrophoresis.Genotype and gene frequency were generally simillar,the polymorphism was found only in Est.Amy-I and Tf.The avarage heterozygosity(H)is 0.0262,the proportion of polymorphic loci(P)is 0.068 2 and the mean number of alleles(A)is 1.0909.The results revealed that the Mithun was depauperate in genetic diversity from the point of view of enzyme gene.Thus the Mithun may have encountered a severe bottleneck in population size,possibly associated with the initial founding event in history.As far as the current status under natural conditions of the Mithun is concerned we discussed the breeding and conservation of the Mithun preliminarily in the view of genetic management.
基金
云南省应用基础研究重点项目
