EB病毒壳抗原在大肠杆菌中的表达及纯化

Expression of the Epstein-Barr Virus P150 Viral Capsid Antigen in Escherichia Coli for the Use as Antigen in Diagnostic Tests

本文报告对EB病毒壳抗原(VCA)带有抗原决定簇的蛋白质在大肠杆菌中的表达及其纯化。以纯化后的蛋白为抗原检测人血清中VCA/IgA抗体,鼻咽癌病人及某些急性EB病毒感染人群呈抗体阳性.而对照组呈阴性。这一结果提示VCA蛋白在鼻咽癌的普查中有应用价值。

We have attempted to produce the P150 viral capsid antigen of Epstein-Barr Virus (EBV) in Escherichia coli. This protein was found, by immun-oprecipitation, to be a clinically relevant antigen, especially for the determination of the IgA-titer in patients with Naso Pharyngeal Carcinoma(NPC).Since the expression of the entire P150 coding region was unsuccessful, we synthesized only the antigenic parts of this protein. After purification of P150 expressed by PUR290CXH580 with column chromatography (sepharose 2B-CL), the resulting product reacts particularly well with IgA antibodies of NPC patients indicating its diagnostic value for NPC.