摘要
高效毛细管电泳(HighPerformanceCapillaryElectrophoresis,简称HPCE))技术近十年来得到了迅速的发展。由于它具有进样量小(nl级)、灵敏度高(10-10mol)、分辨率为百万理论塔板数(高出HPLC数十倍),而且可以快速、高效地分离分子量达106-1011道尔顿的生物大分子等特点,因此受到了化学、生命科学及药物分析、环保等领域的极大量视[1~8]。目前,国外已出现了近十种商品化仪器,而本文介绍的新型毛细管电泳仪是我国第一台商品化仪器,采取了两种进样方法、两种毛细管冲洗方式、多波长UV检测、恒温及微机数据处理系统。本文简要说明了这种仪器的原理、结构和特征,并给出了分离蛋白质、核苷酸及单糖的实验条件和图谱。
During the past 10 years,major progress in the technology of high perfor-mance capillary electrophoresis(HPCE) has taken place. Because of a small sample volumeinjected(nl),high sensitive detection (10-10 mol),high plate efficiencies(up to a million)and short analysis time for biomacromolecule(M =106-1011),HPCE shows strong potentialto be widely used in chemistry,biological science,drug analysis and environmental protec-tion, Now several equipments already appear overseas,In this paper,the HPCE instrumentis one of the first HPCE equipment of our country.It has two types of injection and flush-ing,multi -wavelength UV detecor,data processing system and Constant temperature con-trol.Furthermore,the new type HPCE instrument plays an important role in theory and ap-plication study of HPCE technology in our country. In this paper,the separation results of proteins,nucleotides and monosaccharides withthe new type HPCE instrument are introduced.
出处
《仪器仪表学报》
EI
CAS
CSCD
北大核心
1995年第4期408-413,共6页
Chinese Journal of Scientific Instrument
关键词
毛细管电泳技术
电泳仪
分离检测
Capillary Electrophoresis Tecknology, ElectrophOresis Instrument,Analysis,Detector