新型高效毛细管电泳仪的研究

Study on High Performance Capillary Electrophoresis Chromatography

高效毛细管电泳（ＨｉｇｈＰｅｒｆｏｒｍａｎｃｅＣａｐｉｌｌａｒｙＥｌｅｃｔｒｏｐｈｏｒｅｓｉｓ，简称ＨＰＣＥ））技术近十年来得到了迅速的发展。由于它具有进样量小（ｎｌ级）、灵敏度高（１０－１０ｍｏｌ）、分辨率为百万理论塔板数（高出ＨＰＬＣ数十倍），而且可以快速、高效地分离分子量达１０６－１０１１道尔顿的生物大分子等特点，因此受到了化学、生命科学及药物分析、环保等领域的极大量视［１～８］。目前，国外已出现了近十种商品化仪器，而本文介绍的新型毛细管电泳仪是我国第一台商品化仪器，采取了两种进样方法、两种毛细管冲洗方式、多波长ＵＶ检测、恒温及微机数据处理系统。本文简要说明了这种仪器的原理、结构和特征，并给出了分离蛋白质、核苷酸及单糖的实验条件和图谱。

During the past 10 years,major progress in the technology of high perfor-mance capillary electrophoresis(HPCE) has taken place. Because of a small sample volumeinjected(nl),high sensitive detection (10-10 mol),high plate efficiencies(up to a million)and short analysis time for biomacromolecule(M =106-1011),HPCE shows strong potentialto be widely used in chemistry,biological science,drug analysis and environmental protec-tion, Now several equipments already appear overseas,In this paper,the HPCE instrumentis one of the first HPCE equipment of our country.It has two types of injection and flush-ing,multi -wavelength UV detecor,data processing system and Constant temperature con-trol.Furthermore,the new type HPCE instrument plays an important role in theory and ap-plication study of HPCE technology in our country. In this paper,the separation results of proteins,nucleotides and monosaccharides withthe new type HPCE instrument are introduced.