小鼠子宫颈癌爪垫皮下移植后侵袭程度和转移间的关系

Study on the Metastatic Mechanism and Invasive Degree of Tumor Cells after Subcutaneous Transplantation of Uterine Cervix Carcinoma Cells into Footpad of Mouse

将小鼠子宫颈癌14号瘤细胞移植于小鼠爪垫皮下,在不同间隔时间切除一批荷瘤动物的后下肢(含原发瘤),所有小鼠均于瘤细胞接种后26d处死。观察肿瘤在局部的侵袭程度及肺,淋巴结出现转移的时间及程度.结果表明,瘤细胞在移植后2d开始侵袭,3和5d侵袭达Ⅱ～Ⅲ级,7、9和11d侵袭达Ⅲ～Ⅳ级。肿瘤移植后7d出现淋巴结转移,9d发生肺转移。11d肺和淋巴结转移占80％。可见局部肿瘤达到Ⅲ级侵袭方出现转移。

After mouse ascitic carcinoma cells (U14) 2×106 were inoculated subcu-taneously into right footpads of inbred 615-strain mice, the subcutaneous primary tumor growth was resected by right hind leg amputation in 6-10 mice 1,2,3,5,7,9 and 11 days after inoculation respectively. Another 9 mice without hind leg amputation served as controls. All animals were sacrificed on the 26th day. Results showed that tumor invasion was not apparent on the 1st day after transplantation (grade 0). Slight tumor invasion was noticed on the 2nd day (grade Ⅰ); grades Ⅱ -Ⅲ invasion were noticed on the 3rd-5th days; and grades Ⅲ-Ⅳ invasion on the 7-Ⅰth days. Lymphatic metastases appeared on the 7th day after transplantation, both lymphatic metastasis and lung metastases occurred in 80% of the mice Ⅰ days after inoculation. The results indicate a close relationship between tumor cell invasion and metastasis, i.e., tumor metastasis was noticed on the 7th day as tumor invasion reached grade Ⅲ. Also, lymphatic metastasis was noticed 2 days earlier than lung metastasis. Results of this experiment provide very useful data for studies on the treatment of tumor invasion and metastasis and the relationship between lymphatic and hematogenic metastasis.