摘要
根据国内生产实际情况,用羊血浆生物检测法,以每1g肠粘膜干固体中肝素的抗凝效价作为收率比较指标,设计了多因素多水平的正交试验,对肝素生产中提取、除杂蛋白及离子交换吸附各生产步骤行了研究和优选。发现原料中来源于细菌的肝素酶对肝素破坏严重。探讨了肝素动态吸附以及肝素钙生产工艺。
With multi-factor orthogonal experimental design, the proceduresof extraction, elimination of protein impurities, and ion exchange adsorption inheparin production were studied to find out optimum conditions.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
1989年第7期302-306,共5页
Chinese Journal of Pharmaceuticals
关键词
肝素
提取
纯化
肝素钙
肠粘膜
heparin, extraction, purification,calcium heparinate,manufacture,mucosa, neparinase
